Review on Application of Nucleic Acid Amplification Techniques in Pharmaceutical Products Analysis

Review on Application of Nucleic Acid Amplification Techniques in Pharmaceutical Products Analysis

Article history: Received on: 19/10/2015 Revised on: 07/11/2015 Accepted on: 20/11/2015 Available online: 27/12/2015 The aim of this study was to describe application of molecular diagnostic tests based on nucleic acid amplification technologies (NAATs) in pharmaceutical product analysis. NAAT have become widely established in clinical microbiology laboratories in recent years, as well as in qu...

Sensitivity and Specificity of Nucleic Acid Sequence-Based Amplification Method for Diagnosis of Cutaneous Leishmaniasis

Abstract Background and Objective: Culture, microscopic method is a gold standard method for identification of Lishmania parasite. The use of Molecular methods such as RT- PCR compared to microscopic methods has a higher sensitivity and specificity however, it is not widely used due to its expensive equipment and the time requested. The use of nucleic acid sequence based amplification (NASBA) ...

Digital Nucleic Acid Amplification on a Slipchip

This presentation will describe a recently developed SlipChip based microfluidic platform to perform digital nucleic acid amplification in a simple and low-cost format. Samples can be introduced into the SlipChip by pipetting without the use of complex instruments, and thousands of nanoliter volume compartments can be generated by simple slipping. A “yes-or-no” digital readout of end-point fluo...

Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis: a systematic review and meta-analysis Running title: Nucleic acid amplification tests for tuberculous pleuritis

Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis: a systematic review and meta-analysis

BACKGROUND Conventional tests for tuberculous pleuritis have several limitations. A variety of new, rapid tests such as nucleic acid amplification tests--including polymerase chain reaction--have been evaluated in recent times. We conducted a systematic review to determine the accuracy of nucleic acid amplification (NAA) tests in the diagnosis of tuberculous pleuritis. METHODS A systematic re...